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| Petit, Vanessa W.; Rolland, Jean-luc; Blond, Alain; Cazevieille, Chantal; Djediat, Chakib; Peduzzi, Jean; Goulard, Christophe; Bachere, Evelyne; Dupont, Joelle; Destoumieux-garzon, Delphine; Rebuffat, Sylvie. |
| Background. Hemocyanins are respiratory proteins with multiple functions. In diverse crustaceans hemocyanins can release histidine-rich antimicrobial peptides in response to microbial challenge. In penaeid shrimp, strictly antifungal peptides are released from the C-terminus of hemocyanins. Methods. The three-dimensional structure of the antifungal peptide PvHCt from Litopenaeus vannamei was determined by NMR. Its mechanism of action against the shrimp pathogen Fusarium oxysporum was investigated using immunochemistry, fluorescence and transmission electron microscopy. Results. PvHCt folded into an amphipathic α-helix in membrane-mimicking media and displayed a random conformation in aqueous environment. In contact with F. oxysporum, PvHCt bound... |
| Tipo: Text |
Palavras-chave: Antimicrobial peptide; Amphipathic helix; Fungi; Membrane bilayer; Nuclear magnetic resonance (NMR); Fluorescence microscopy. |
| Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00301/41202/40362.pdf |
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| Van Dijk, Juliette; Bompard, Guillaume; Cau, Julien; Kunishima, Shinji; Rabeharivelo, Gabriel; Mateos-langerak, Julio; Cazevieille, Chantal; Cavelier, Patricia; Boizet-bonhoure, Brigitte; Delsert, Claude; Morin, Nathalie. |
| Background Upon maturation in the bone marrow, polyploid megakaryocytes elongate very long and thin cytoplasmic branches called proplatelets. Proplatelets enter the sinusoids blood vessels in which platelets are ultimately released. Microtubule dynamics, bundling, sliding, and coiling, drive these dramatic morphological changes whose regulation remains poorly understood. Microtubule properties are defined by tubulin isotype composition and post-translational modification patterns. It remains unknown whether microtubule post-translational modifications occur in proplatelets and if so, whether they contribute to platelet formation. Results Here, we show that in proplatelets from mouse megakaryocytes, microtubules are both acetylated and polyglutamylated. To... |
| Tipo: Text |
Palavras-chave: Microtubules; Acetylation; Polyglutamylation; Tubulin isotype; Platelets; Megakaryocytes; IIb3 integrin; CHO cells. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00463/57463/59577.pdf |
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| Robino, Etienne; Poirier, Aurore C.; Amraoui, Hajar; Le Bissonnais, Sandra; Perret, Angelique; Lopez‐joven, Carmen; Auguet, Jean-christophe; Rubio, Tristan P.; Cazevieille, Chantal; Rolland, Jean-luc; Héchard, Yann; Destoumieux‐garzón, Delphine; Charrière, Guillaume. |
| ibrios are ubiquitous in marine environments and opportunistically colonize a broad range of hosts. Strains of Vibrio tasmaniensis present in oyster farms can thrive in oysters during juvenile mortality events and behave as facultative intracellular pathogen of oyster hemocytes. Herein, we wondered whether V. tasmaniensis LGP32 resistance to phagocytosis is specific to oyster immune cells or contributes to resistance to other phagocytes, like marine amoebae. To address this question, we developed an integrative study, from the first description of amoeba diversity in oyster‐farms to the characterization of LGP32 interactions with amoebae. An isolate of the Vannella genus, Vannella sp. AP1411, which was collected from oyster farms, is ubiquitous, and... |
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| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00509/62109/66292.pdf |
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